| a: Prosthetic group is protohematin; b: inhibited by sulfhydryl compounds, KCN, NaN3; 50% inhibition being rpoduced by 5 x 10 to minus 6 M KCN, 2 x 10-5 NaN3; c: Heat stability, inactivated by 10 min of incubation at 60C but is indefinitely stable at 1 degree between pH 5.3 and 8.9; d: Optimum activity is between pH 5.3 and 8.0, falling off rapidly at more acid values and slowly at more alkaline values. |
Beef Liver Catalase
| isoelectric point is pH 5.7 |
Catalases
| Have visible absorption bands; b: Is one of the most powerful enzymes known; one molecule decomposes 2.6 x 10 to the six power molecules H2O2 per min at 0C. |
QUALITATIVE TEST: Various methods for testing for catalase activity have been used. The following method is simple and indicates the presence or absence of catalase. Place about 2 grams of well mixed, coarsely broken plant ittue in a large test tube with 20 mL of distilled water. Soak 15 min, then add 0.5 mil of 0.5% H2O2 solution. If catalase is still active, a fairly vigorous evolution of O2 will ensue within a few minutes.
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´ ´ Updated: Wednesday, October 31, 2007.
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