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Browning |
Banana slices were cut to weigh 10.00 g. The control slices were placed in 20 ml of 1 percent thiourea which stopped the enzymatic browning reaction. The other slices were placed in 20 ml of distilled water. The tissue was homogenized in a microblender and filered in a Buchner funnel using Whatman No. 1 filer paper.
The extent and the susceptibility to browning were measured by the method of Gaudagni et al. (1949). One ml of filtrate was added to 5 ml of water in a cuvette and optical density was read at 475 nm with a spectrophotometer (Spectronic-20 Spectrophotometer, Bausch and Lomb Inc., Analytical Systems Division, Linden Ave., Rochester, New York 14625). Readings were taken periodically over a 30-minute period and curves were plotted as percent transmittance against time.
Updated: Wednesday, June 20, 2007.
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