ENZYMES

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Amylases

Pectinase

Peroxidase

Rennet

FREQUENTLY ASKED QUESTIONS to Top

How does rennet function?
How are enzymes named?
What is an enzyme?
What gives bread its light, fluffy texture?
What is the source and applications of some industrial enzymes?
LINKS to Top

http://www.brenda.uni-koeln.de/
Brenda is the Comprehensive Enzyme Information System. Has the function, structure, and activity of enzymes. Here finds what reaction the enzyme catalyzes and what compounds inhibit or activate it; where in the cell it works; the optimum temperature and pH for the reaction; how to store a sample; and whether it is implicated in any diseases. Breanda is free with registration for academics and researchers at nonprofit organizations.

http://www.enzymedevelopment.com/
Enzyme Development Corporation has enzyme preparations for sale with comments and specifications. Includes links to sites of interest.

http://www.enzymesys.com/
Enzyme Systems Products has information and products for beta and gamma secretase.

http://www.novo.dk/
The History of Enzyme Technology

http://ingredients.danisco.com/
Danisco Cultor has enzymes, emulsifiers and enzymes.

http://www.dyadic-group.com/
Dyadic International Inc. is a leader in the research, development, manufacturing, and marketing of industrial enzymes, minerals, chemical formulations, and processing aids for the natural fiber and food processing industries

http://www.megazyme.com/
Megazyme has enzymes and Diagnostic Kits and Reagents has many test kits and reagents. Works in baking, textiles, research, brewing, analytical laboratory, plant breeding, fermentation, detergents, milling and animal feed.

http://www.restrictionmapper.org/
Restriction Mapper is a program that tells you which enzymes will snip the DNA strand and where, and then it supplies the sequences of the fragments. It has links to other free molecular biology software, including programs for sequence alignment and gene annotation.

http://ValleyEnzymes.com/
Valley Research, Inc. has been providing enzymes and technical service to the food, fruit and vegetable processing, baking, beverage, alcohol, and other related industries since 1984. Site includes products, customer server, application research, enzymes, cultures and what is new.

INFORMATION to Top

FoodEnzymes Influence/Effect
pears polyphenolxidase Bartlett pear enzyme catalyzed oxidative browning of plant tissues and decreases quality of product. Acts on substrates of catechol, chlorogenic acid, caffeic acid, D-catechin, protocathechuric acid.
pears indole-3-acetic acid oxidase Controls ripening of pear in cooperative manner
pear peroxidase It works in the developing pear fruit
pear malic enzyme Has a role in the biosynthesis of ripening fruit.
pear chlorophyllase Likely plays a role in degreening of fruit.
pear transaminase Likely plays a role in flavor biogenesis
pectinases
polygalacturonase
exopolygalacturonase
endopolygalacturonase
pectic acid transeliminase
endo-PATE
exo_PATE
Acts on pectin to soften fruits
pear pectinases
pectin methyl esterase (PE)

endo-PMG(acid and alkaline)
Acts on pectin to soften fruits.
pear pectinanse
pectin transeliminase(PTE)
endo-PTE
exo-PTE
Acts on pectin to solften fruits
cheese
cheddar
milk enzymes
xanthin oxidase
Possibly oxidative flavors
cheese
cheddar
milk enzymes
lipase
development of hydrolytic rancidity
cheese
cheddar
milk enzymes
lipase
development of hydrolytic rancidity
cheese
cheddar
milk enzyme
catalase
development of hydrolytic rancidity
cheese
cheddar
milk enzyme
peroxidase
probably inactivated by pasteurization
cheese
cheddar
milk enzymes
phosphatase
cheese
cheddar
milk enzymes
protease
cheese
cheddar
cheddar producing
crude rennet-pepsin extract
contributes to proteolytis of proteins. May possibly develop bitterness in cheese.
cheese
cheddar
cheddar producing
protease
cheese
cheddar
cheddar producing
rennin
cheese
cheddar
cheddar producing
pepsin
cheese
cheddar
cheddar producing
chymotripsin
cheese
cheddar
cheddar producing
papain
cheese
cheddar
cheddar producing
trypsin
cheese
cheddar
cheddar producing
lipase
possibly enhances butyric acid development
cheese
cheddar
cheddar producing
citrate metabolizing species
L. citrovorum
Contributes broad spectrum of enzymes flavor and texture
cheese
cheddar
cheddar producing
deaminases, decarboxylases, aminases, transaminases
Likely contribute to development of flavors
chocolate
covered
cherries
invertaseDecreases crystallinity of sucrose by hydrolysis of sucrose to invert sugars fructose and glucose.
chocolate
covered
cherries
cherry plant enzymesMany of those mentioned under the section on pears.

AMYLASES to Top

Zondagh, I.B.. 1983, Spring. Chicken Broth Project. FN531. dept. Foods and Nutrition, Oregon State University, Corvallis, OR 97330.
REFERENCES to Top

Abdullah, M.; Catley, B. J.; Lee, E. Y. C.; Robyt, J.; Wallenfels, K.; Whelan, W. J. 1966. The Mechanism Of Carbohydrase Action. Ii. Pullulanase, An Enzyme Specific For The Hydrolysis Of Alpha-1-6 Bonds In Amylaceous Ogligo- And Polysaccharides. Cereal Chemistry 43:111
Pullulanase, Polysaccharide, Carbohydrase, Oligosaccharide

Anderson, J.E., D.M. Adams, W.M. Walter Jr. 1983. Conditions under which bacterial amylases survive ultrahigh temperature sterilization. Journal Food Science 48: 1622.

Asp, E.H. 1990. Laboratory methods for alpha-amylase analysis. Cereal Foods World 35(9): 941.

Bache, E. D. 1916. Rennet Custard - The Dish That Makes Itself. Journal of Home Economics 8:195
Rennet Custard

Derosier, N. W. ; Mcardle, F. J.; Hollender, H. A.; Marion, W.W. 1955. What Cathode Rays Do To Eggs And Milk. Food Engineering 27(5):78
> Cathode Ray, Irradiated Milk, Irradiated Egg, Sensory Evaluation, Egg, Flavor, Milk, Rennet, Coagulation Time

Deshpande, S.S. and M. Cheryan. 1984. Effects of phytic acid, divalent cations, and their interactions on alpha-amylase activity. journal of Food Science 49: 516.

Deshpande, S.S., S.K. Sathe, D. Cornforth, and D.K. Salunkhe. 1982. Cereal Chemistry 59(5): 396-401.

French, D.; Robyt, J. F.; Weintraub, M.; Knock, P. 1966. Separation Of Maltodextrins By Charcoal Chromatography. Journal Of Chromatography 24:68
Amylose, Alpha-Amylase, Oligosaccharide, Waxy Corn Starch, Aspergillus Oryzae, Salivary

Innis, M.A., M.J. Holland, P.C. McCabe, G.E. Cole, V.P. Wittman, R. Tal, K.W.K. Watt, D.H. Gelfand, j.p. Holland, J.H. Meade. 1985april 5. Expression, glycosylation, and secretion of an Aspergillus glucoamylase by ,i>Saccharomyces cerevisiae. Science 228: 21.

Jones, B.L. and P. Meredith. 1982(4). Inactivation of alpha-amylase activity by purothionins. Cereal Chemistry 59(4): 321.

Kalab, M.; Harwalkar, V. R. 1973. Milk Gel Structure. I. Application Of Scanning Electron Microscopy To Milk And Other Food Gels. Journal Of Dairy Science 56:835
Tofu, Egg White, Egg, Gel, Gelatin Gel, Milk, Gel, Rennet, Electron Microscopy

Kopelman, I. J.; Cogan, U. 1976. Determination Of Clotting Power Of Milk Clotting Enzymes. Journal Of Dairy Science 59:196
Milk Clotting Enzyme, Rennet, Macor Pusillus, Endothia Parasitica

Kowalchyk, A. W.; Olson, N. F. 1977. Effects Of Ph And Temperature On The Secondary Phase Of Milk Clotting By Rennet. Journal Of Dairy Science 60:1236
Rennet, Brookfield Viscometer, Milk Coagulation

Ledford, R. A.; Chen, J. H.; Nath, K. R. 1968. Degradation Of Casein Fractions By Rennet Extract. Journal Of Dairy Science 51:792
Proteolytic Enzyme, Rennet, Casein, Electrophoresis

Lineback, D.R., I.J. Russell, C. Rasmussen. 1969. Two forms of the glucoamylase of Aspergillus niger. Archives of Biochemistry and Biophysics 134: 539-553.

Marchylo, b.A. and A.W. Macgregor. 1983. Separation of barley malt alpha-amylase by chromatofocusing. Cereal Chemistry 60(4): 311-314.

Murty, D.S., V. Subramanian, s. Suryaprakash, H.D. Patil, and L.R. House. 1984. Amylase activity and sprout damage in sorghum (Sorghum bicolor L. Moench). Cereal Chemistry 61(5): 415-418

Nagaraj, R.H. and T.N. pattabiraman. 1985. Isolation of an amylase inhibitor from Setaria italica grains by affinity chromatography on blue-sepharose and its characterization. Journal Agric. Food Chem. 33: 646-650.

Okon, E.U. and A.O. Uwaifo. 1984. Partial purificaton and properties of beta-amylase isolated from Sorghum bicolor (L.) Moench. Journal Agric. Food Chemistry 32:11-14.

Patel, M. C.; Lund, D. B.; Olson, N. F. 1972. Factors Affecting Syneresis Of Renneted Milk Gels. Journal Of Dairy Science 55:913
Rennet, Milk, Gel

Pazur, J. H.; Sandstedt, R. M. 1954. Identification Of The Reducing Sugars In Amylolysates Of Starch And Starch- Oligosaccharides Cereal Chemsitry 31:416
Starch, Oligosaccharide, Starch, Reducing Sugar, Amylolysate

Roberts, H. R.; Pettinati, J. D. 1957. Oligosaccharide Production, Concentration Effects In The Enzymatic Conversion Of Lactose To Oligosaccharides. . Journal Of Agricultural And Food Chemistry 5:130
Oligosaccharide, Enzyme Conversion, Lactose

Sandstedt, R.M., E. Kneen, and M.J. Blish. 1939. A standardized wohlgemuth procedure for alpha-amylase activity. Cereal Chemistry 16: 712-723.

Schacht, F. L.; Nichols, R. E. 1941. Studies Of The Resazurin-Rennet Test. Journal Of Milk Technology 4:281
> Resazurin-Rennet Test, Milk Odor, Milk Bacteria, Methylene Blue Reduction Test, Resazurin Test

Sim, S.K., S.M. Ohmann, and C.B.S. Tong. 1997. Comprison of polyphenol oxidase in tubers of Solanum Tuberosum and the non-browning tubers of S. Hjertingii
Stamberg, O. E.; Bailey, C. H. 1942. The Effect Of Heat Treatment Of Milk In Relation To Baking Quality As Shown By Polarograph And Farinograph Studies. Cereal Chemistry 19:507
Farinograph, Polarography, Milk, Rennet

Thomas, A.A., T.P. Drozd, and T.J. Walter. 1984. Amylase activities in fungal rennets and whey protein concentrate powder. Journal Agricultural and Food Chemistry 32: 825.

Thompson, L.U. and J. E. Gabon. 1987. Effect of lectins on salivary and pancreatic amylase activities and the rate of starch digestion. Journal Food Science 52(4): 1050.

Towsend, W.E. and L.C. Blankenship. 1987. Assessment of previous heat treatment of laboratory heat-processed meat and poultry using a commercial enzyme system. Journal Food Science 52(6): 1445-1448.

Walter, W.M. Jr. and A.E. purcell. 1973. Alpha-amylase in sweet potatoes. A comparison between the amyloclastic and chromogenic starch methods of analysis. Journal Food Science 38: 548.

Wasserman, B.P. 1984. Thermostable enzyme production. Food Technology 38(2): 78.

Watson, C.A. 1984. Instruments for determining alpha-amylase activity. Cereal Foods World 29(9): 567.

Weckel, K. G.; Jackson, H. C. 1936. Observations On The Source Of Flavor In Milk Exposed For Prolonged Periods To Radiation. Food Research 1:419
Ultraviolet Radiation, Vitamin D, Radiation, Pasteurization, Heat, Antirachitic Agent, Milk, Dairy Product, Cream, Skim Milk, Buttermilk, Butteroil, Casein, Oil, Albumin, Whey, Whey Filtrate, Protein, Light Transmission, Spectroscopy, Quartz, Glass, Flavor Tallowy, Flavor Oily, Lactose, Rennet, Casein, Rennet-Casein, Whole Milk, Aroma, Lactalbumin, Flavor Oxidized

Wegner, E. S.; Jordan, R.; Hollender, H. A. 1953. Homogenized And Nonhomogenized Milk In The Preparation Of Selected Food Products. Journal Of Home Economics 45:589.
Texture, Rennet Dessert, Flavor, Consistency, Vanilla Pudding, Butterscotch Pudding, Chocolate Pudding, Penetrometer, Triangle Test, Sedimentation, Cocoa Beverage, Homogenized Milk, Milk, Nonhomogenized Milk, Chocolate Milk, Palatability, Body

Weselake, R.J., A.W. MacGregor and R.D. Hill. 1985. Endogenous alpha-amyloase inhibitor in various cereals. Cereal Chem. 62(2): 120-123.
Weselake, R.J., A.W. Macgregor and R.D. Hill. 1985. Effect of endogenous barley alpha-amylase inhibitor on hydrolysis of starch under various conditions. Journal Cereal Science 3: 249-259.

West, S. 1988(April). The enzyme maze. Food Technology :98.

Yapa, P.A.J., T. Kawasaki, and H. Matsumoto. 1986. Changes of some membrane-associated enzyme activities and degradation of membrane phospholipids in cucumber roots due to Ca2+ starvation. Plant Cell Physiol. 27(2): 223-232.

Zeman, N.W. and J.M. McCrea. 1985. Alpha-amylase production using a recombinant DNA organism. Cereal Foods World 30: 777.

Updated: Wednesday, October 24, 2007.

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