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Type of Enzyme

Carbohydrase

Depolymerase

Poly-alpha-1,4 galacturonide, pectin pectylhydrolase

IUB #: 3.2.1.15, 3.1.1.11

Mode of Action:

Hydrolyzes the linkages that hold the small building blocks of galacturonic acid together in the pectic substances, producing smaller molecules.

Influence:

1. Optimum temperature and pH values vary in sources.

e.g. mold origin: deactivation at 60C, pH3.0
Penicillium: 40C, pH 3.0-3.5

2. The presence of calcium or other heavy metal cations cause an apparent differences of enzyme activity. Tannin also tends to reduce the activity of enzymes.

Functions:
1. Clarifies apple juice.
2. Reduces viscosity by hydrolyzing the pectin.
3. Accelerates rate of filtration
4. Prevents pectin gel formation of juice from the fruit, thus increasing yield.
5. Improve color extraction from grape skin.
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Updated: Wednesday, October 24, 2007.

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